YCplac22 (ATCC® 87585)

Applications: YC-type (centromeric) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

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Designations YCplac22
Depositors RD Gietz
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 4.8540000915527340
DESCRIPTION OF VECTOR:
Intact vector size: 4.854
Type of vector: plasmid
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Other unique sites: EcoRV XcaI AatII NarI SpeI NaeI NheI BglII
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: lac, ->, 143-171
insert detection: lacZ', ->, 216-500
MCS: HindIII...EcoRI, ->, 234-285
centromere: CEN4, 504-1480
replicon: ARS1, 1481-2225
marker(s): TRP1, <-, 2063-2740
marker(s): ampR, ->, 3053-3913
replicon: pMB1, 4666-4666
Vector: YCplac22 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):TRP1,ampR
Construct size (kb): 4.854000091552734
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: HindIII...EcoRI
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--2.9, 1.9; HindIII--4.8; XbaI--4.8.
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
The HindIII, PstI and the XbaI sites from the TRP1 gene of the S. cerevisiae were removed by using oligo-directed mutagenesis.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

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Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87585