pHT6 [HIS3 --> TRP1 converter] (ATCC® 87543)

Applications: YI-type (integrating) shuttle vectorhost modificationmarker swap vector HIS3 --> TRP1  /  Depositors: FR Cross

Permits and Restrictions

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Designations pHT6 [HIS3 --> TRP1 converter]
Depositors FR Cross
Biosafety Level 1
Vector Information
Size (kb): 7.0000000000000000
Vector: pHT6 (phagemid)
Construction: pBC KS+
Marker(s):TRP1,cmlR,kanR
Construct size (kb): 7.0
Features: gene disruption cassette: his3::TRP1/kanR
marker(s): cmlR
replicon: f1
replicon: pMB1
restriction site: PstI, SmaI, PstI
restriction site: XhoI, SalI
Applications
YI-type (integrating) shuttle vector
host modification
marker swap vector HIS3 --> TRP1
Comments
To convert the host phenotype from HIS3 to TRP1, transform with the SmaI+XhoI digested vector and select for Trp+ transformants.
Some combinations of marker swap plasmids and target locus may result in relatively high reversion rates. In most but not all cases the frequencies of successful convertants are greater than 30%.
A marker swap vector designed to change the S. cerevisiae host phenotype by one-step gene disruption of the HIS3 gene with the TRP1 and kanR markers.
When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance.
Vector was constructed by replacing an internal HindIII fragment of HIS3 with a SmaI fragment containing the TPR1 and kanR coding sequences. TRP1 and HIS3 are in the same orientation.
Media
Growth Conditions
Temperature: 37.0°C
References

Cross FR. 'Marker swap' plasmids: convenient tools for budding yeast molecular genetics. Yeast 13: 647-653, 1997. PubMed: 9200814

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component of:ATCC 87561
Shipped freeze-dried
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