pRS416 (ATCC® 87521)

Applications: YC-type (centromeric) shuttle vector  /  Depositors: P Hieter

Permits and Restrictions

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Designations pRS416
Depositors P Hieter
Biosafety Level 1
Host
Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 4.898
DESCRIPTION OF VECTOR:
Intact vector size: 4.898
Type of vector: phagemid
Cloning sites: KpnI ApaI XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI EagI
NotI SacII SacI NaeI
Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI
SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII
Construction: pBluescriptII KS+
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 417-1219
replicon: f1, <-, 1351-1807
insert detection: lacZ', <-, 1800-2137
promoter for in vitro transcription: T7, ->, 1977-1996
MCS: KpnI...SacI, ->, 2004-2111
promoter for in vitro transcription: T3, <-, 2123-2142
promoter: lac, <-, 2212-2240
replicon: pMB1, 2566-2566
marker(s): ampR, <-, 3324-4184
centromere: CEN6, 4326-4442
replicon: ARSH4, 4443-4806
Vector: pRS416 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Marker(s):URA3,ampR
Construct size (kb): 4.898
Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--4.45, 0.45; BglI--3.2, 1.7; PstI--3.25, 1.65.
A YC-type shuttle vector with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

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component of:ATCC 87534
Shipped frozen
Shipping Information Distributed: freeze-dried
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