pKB112 (ATCC® 87512)

Applications: YE-type (episomal) shuttle vector  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations pKB112
Depositors DJ Stillman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 10.1999998092651400
Vector: pKB112 (plasmid)
Promoters: Promoter CYC1 promoter (including CYC1 UAS)
Construction: pBR322
Construct size (kb): 10.19999980926514
Features: marker(s): URA3
marker(s): ampR
promoter: CYC1 promoter (including CYC1 UAS)
replicon: 2 micron ori
replicon: pMB1
coding sequence: lacZ
YE-type (episomal) shuttle vector
Restriction digests of the clone give the following sizes (kb): KpnI--10.3; HindIII--8.2, 2.1; SalI--10.3.
This vector is one of three lacZ reporter plasmids that contain UAS (upstream activation site) elements (ATCC 87511, 87512 & 87522). They differ by polylinker sites.
DNA fragments can be inserted into these plasmids to screen for DNA elements that reduce promoter activity.
The vector was constructed by Aaron Mitchell.
Growth Conditions
Temperature: 37.0°C

Bowdish KS, Mitchell AP. Bipartite structure of an early meiotic upstream activation sequence from Saccharomyces cerevisiae. Mol. Cell. Biol. 13: 2172-2181, 1993. PubMed: 8455605

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