pLGdelta312 (ATCC® 87511)

Applications: YE-type (episomal) shuttle vector  /  Depositors: DJ Stillman

Designations pLGdelta312
Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 10.1999998092651400
Vector: pLGdelta312 (plasmid)
Promoters: Promoter CYC1 promoter (including the CYC1 UAS)
Construction: pBR322
Marker(s):URA3,ampR
Construct size (kb): 10.19999980926514
Features: marker(s): URA3
marker(s): ampR
promoter: CYC1 promoter (including the CYC1 UAS)
replicon: 2 micron ori
replicon: pMB1
coding sequence: lacZ
Applications
YE-type (episomal) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--10.3; HindIII--8.0, 2.1; EcoRI--4.5, 3.15, 2.25.
This vector is one of three lacZ reporter plasmids that contain UAS (upstream activation site) elements (ATCC 87511, 87512 & 87522). They differ by polylinker sites.
DNA fragments can be inserted into these plasmids to screen for DNA elements that reduce promoter activity.
The vector was constructed by Lenny Guarente.
Growth Conditions
Temperature: 37.0°C
References

Guarente L, Hoar E. Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA" box. Proc. Natl. Acad. Sci. USA 81: 7860-7864, 1984. PubMed: 6096863

Related Products
component of:ATCC 87540
Shipped freeze-dried
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