pLGdelta178 (ATCC® 87509)

Applications: YE-type (episomal) shuttle vector  /  Depositors: DJ Stillman

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Designations pLGdelta178
Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 10.0600004196167000
Vector: pLGdelta178 (plasmid)
Promoters: Promoter truncated CYC1 promoter (no UAS element)
Construction: pBR322
Marker(s):URA3,ampR
Construct size (kb): 10.0600004196167
Features: marker(s): URA3
marker(s): ampR
promoter: truncated CYC1 promoter (no UAS element)
replicon: 2 micron ori
replicon: pMB1
coding sequence: lacZ
Applications
YE-type (episomal) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): XhoI--11.0; HindIII--8.7, 2.2; EcoRI--4.75, 3.45, 2.4.
This is one of three lacZ reporter plasmids that lack UAS promoter elements (ATCC 87509, 87510 & 87513). They differ by polylinker sites.
DNA fragments can be inserted into these plasmids to assay UAS (upstream activation site) activity.
The vector was constructed by Lenny Guarente.
Growth Conditions
Temperature: 37.0°C
References

Guarente L, et al. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae. Cell 36: 503-511, 1984. PubMed: 6319028

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component of:ATCC 87539
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