p425 CYC1 (ATCC® 87383)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p425 CYC1
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 7.3670001029968260
DESCRIPTION OF VECTOR:
Intact vector size: 7.367
Type of vector: plasmid
Cloning sites: SalI HindIII PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI
XbaI_
Other unique sites: SacI
Construction: pRS425
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: SalI...XbaI, ->, 3394-3452
promoter for expression: CYC1, <-, 3452-3739
marker(s): ampR, <-, 4870-5800
replicon: 2 micron, ->, 5920-7260
Vector: p425 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS425
Marker(s):LEU2,ampR
Construct size (kb): 7.367000102996826
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CYC1
replicon: 2 micron
MCS: SalI...XbaI
terminator: CYC1
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9, 3.2, 0.35; XbaI--3.9, 3.5; KpnI--6.0, 1.4.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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