p415 CYC1 (ATCC® 87382)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p415 CYC1
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.5339999198913570
Intact vector size: 6.534
Type of vector: plasmid
Cloning sites: SalI HindIII PstI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI
Other unique sites: SacI
Construction: pRS415
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: SalI...XbaI, ->, 3394-3452
promoter for expression: CYC1, <-, 3452-3739
marker(s): ampR, <-, 4986-5916
replicon: CEN6/ARSH4, ->, 6000-6514
Vector: p415 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS415
Construct size (kb): 6.533999919891357
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CYC1
replicon: CEN6/ARSH4
MCS: SalI...XbaI
terminator: CYC1
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--6.2, 0.3; KpnI--5.0, 1.6; EcoRI--4.3, 2.3.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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