p415 CYC1 (ATCC® 87382)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p415 CYC1
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.5339999198913570
DESCRIPTION OF VECTOR:
Intact vector size: 6.534
Type of vector: plasmid
Cloning sites: SalI HindIII PstI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI
XbaI_
Other unique sites: SacI
Construction: pRS415
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: SalI...XbaI, ->, 3394-3452
promoter for expression: CYC1, <-, 3452-3739
marker(s): ampR, <-, 4986-5916
replicon: CEN6/ARSH4, ->, 6000-6514
Vector: p415 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS415
Marker(s):LEU2,ampR
Construct size (kb): 6.533999919891357
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CYC1
replicon: CEN6/ARSH4
MCS: SalI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--6.2, 0.3; KpnI--5.0, 1.6; EcoRI--4.3, 2.3.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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