p423 CYC1 (ATCC® 87379)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

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Designations p423 CYC1
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 6.3159999847412110
Vector: p423 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS423
Marker(s):ampR,HIS3
Construct size (kb): 6.315999984741211
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: CYC1
replicon: 2 micron
MCS: SalI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.35; XbaI--3.4, 2.9; HindIII--4.8, 1.4.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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