p423 CYC1 (ATCC® 87379)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p423 CYC1
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.3159999847412110
Vector: p423 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS423
Construct size (kb): 6.315999984741211
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: CYC1
replicon: 2 micron
MCS: SalI...XbaI
terminator: CYC1
YX-type (expression) shuttle vector
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.35; XbaI--3.4, 2.9; HindIII--4.8, 1.4.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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