p424 ADH (ATCC® 87373)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p424 ADH
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 7.3470001220703130
Vector: p424 ADH (plasmid)
Promoters: Promoter for expression ADH
Construction: pRS424
Marker(s):TRP1,ampR
Construct size (kb): 7.347000122070313
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: ADH
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.4; EcoRI--7.4; SacI/XbaI--3.2, 1.7, 1.5, 1.0.
The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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Product Sheet
Attachments Vector map for 87373