p426 GPD (ATCC® 87361)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Designations p426 GPD
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.6370000839233400
DESCRIPTION OF VECTOR:
Intact vector size: 6.637
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS426 (ATCC 77107)
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1300
terminator: CYC1, ->, 2004-2264
MCS: XhoI...XbaI, ->, 2265-2339
promoter for expression: GPD, <-, 2339-3019
marker(s): ampR, <-, 4138-5068
replicon: 2 micron, ->, 5167-6507
Vector: p426 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS426 (ATCC 77107)
Marker(s):URA3,ampR
Construct size (kb): 6.63700008392334
Features: marker(s): URA3
marker(s): ampR
promoter for expression: GPD
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.7; EcoRI--6.6; BamHI--6.6.
High copy number shuttle expression vector.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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