p425 GPD (ATCC® 87359)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p425 GPD
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host Escherichia coli HB101 (ATCC 33694)
Vector Information
Intact vector size: 7.76
Type of vector: plasmid
Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS425
Host range: Saccharomyces cerevisiae, Candida robusta, Escherichia coli

Features (with orientation and position when available):
marker(s):
LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: XhoI...XbaI, ->, 3388-3452
promoter for expression: GPD, <-, 3452-4132
marker(s): ampR, <-, 5284-6214
replicon: 2 micron, ->, 6313-7653
Vector: p425 GPD (plasmid)
Promoters: Promoter for expression GPD
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9, 3.2, 0.7; EcoRI--5.5, 2.3; BamHI--7.8.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
Component of: ATCC 87669
Shipped frozen
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87359