p425 GPD (ATCC® 87359)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p425 GPD
Depositors M Funk
Biosafety Level 1
Host Escherichia coli HB101 (ATCC 33694)
Vector Information
Intact vector size: 7.76
Type of vector: plasmid
Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS425
Host range: Saccharomyces cerevisiae, Candida robusta, Escherichia coli

Features (with orientation and position when available):
marker(s):
LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: XhoI...XbaI, ->, 3388-3452
promoter for expression: GPD, <-, 3452-4132
marker(s): ampR, <-, 5284-6214
replicon: 2 micron, ->, 6313-7653
Vector: p425 GPD (plasmid)
Promoters: Promoter for expression GPD
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9, 3.2, 0.7; EcoRI--5.5, 2.3; BamHI--7.8.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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Component of: ATCC 87669
Shipped frozen
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Attachments Vector map for 87359