p424 GPD (ATCC® 87357)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p424 GPD
Depositors M Funk
Biosafety Level 1
Host Escherichia coli HB101 (ATCC 33694)
Vector Information
Intact vector size: 6.527
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS424
Host range: Saccharomyces cerevisiae; Candida robusta; Escherichia coli
Features (with orientation and position when available):
replicon: 2 micron, →
marker(s): TRP1, →
terminator: CYC1, →
promoter for expression: GPD, ←
marker(s): ampR, ←
Vector: p424 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS424
Marker(s): TRP1, ampR
Construct size (kb): 6.527
Features:
Marker(s):
TRP1, ampR
Promoter for expression: GPD Replicon: 2 micron
Terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.7, 1.0, 0.68; EcoRI--6.5; BamHI--6.5.
High copy number shuttle expression vector.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37 C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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Component of: ATCC 87669
Shipped frozen