p414 GPD (ATCC® 87356)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p414 GPD
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 5.6939997673034670
Vector: p414 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS414
Marker(s):TRP1,ampR
Construct size (kb): 5.693999767303467
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.9, 0.7; EcoRI--5.8; BamHI--5.8.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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component of:ATCC 87669
Shipped freeze-dried
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Attachments Vector sequence