p414 GPD (ATCC® 87356)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p414 GPD
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.6939997673034670
Vector: p414 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS414
Construct size (kb): 5.693999767303467
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.9, 0.7; EcoRI--5.8; BamHI--5.8.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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component of:ATCC 87669
Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector sequence