p423 GPD (ATCC® 87355)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Designations p423 GPD
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 6.7090001106262210
Vector: p423 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS423
Marker(s):ampR,HIS3
Construct size (kb): 6.709000110626221
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: GPD
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.7; EcoRI--6.7; BamHI--6.7.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped frozen
Product Sheet
Product Sheet
Product Sheet
Attachments Vector sequence