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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.5000000000000000 Vector: pUCP19 (plasmid) Promoters: Promoter for expression lac Construction: pUC19, pRO1614 Marker(s):ampR,CbR Construct size (kb): 4.5 Features: marker(s): ampR, CbR
promoter for expression: lac
replicon: bhr (rep pRO1600)
replicon: pMB1
MCS: EcoRI...HindIII
coding sequence: lacZalpha
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Applications
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expression vector shuttle vector vector containing primer sites useful for sequencing vector permitting construction of fusion proteins vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.5; EcoRI--4.5; HindIII--4.5; EcoRI/PvuII--3.7, 0.47, 0.31, 0.16; HincII--3.9, 0.6; PvuI--2.7, 1.8. Plasmid is maintained in P. aeruginosa at 10-25 copies per cell. Vector allows screening of recombinants on LB XGal indicator medium as well as on MacConkey lactose medium. Shuttle expression vector allowing visual detection of recombinant molecules in a broad host range. Constructed by insertion of a 1.8 kb PstI fragment from pRO1614 into the NarI site of pUC19.
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References
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Karkhoff-Schweizer RR, Schweizer HP. Utilization of a mini-Dlac transposable element to create an alpha-complementation and regulated expression system for cloning in Pseudomonas aeruginosa. Gene 140: 7-15, 1994. PubMed: 8125342
Schweizer HP. Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 97: 109-112, 1991. PubMed: 1899844
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Shipping Information
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 Freeze dried Escherichia coli DH5alphaF’ containing the plasmid.
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