pUCP19 (ATCC® 87110)

Applications: expression vectorshuttle vectorvector containing primer sites useful for sequencingvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: HP Schweizer

Permits and Restrictions

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Designations pUCP19
Depositors HP Schweizer
Biosafety Level 1
Vector Information
Size (kb): 4.5000000000000000
Vector: pUCP19 (plasmid)
Promoters: Promoter for expression lac
Construction: pUC19, pRO1614
Marker(s):ampR,CbR
Construct size (kb): 4.5
Features: marker(s): ampR, CbR
promoter for expression: lac
replicon: bhr (rep pRO1600)
replicon: pMB1
MCS: EcoRI...HindIII
coding sequence: lacZalpha
Applications
expression vector
shuttle vector
vector containing primer sites useful for sequencing
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.5; EcoRI--4.5; HindIII--4.5; EcoRI/PvuII--3.7, 0.47, 0.31, 0.16; HincII--3.9, 0.6; PvuI--2.7, 1.8.
Plasmid is maintained in P. aeruginosa at 10-25 copies per cell.
Vector allows screening of recombinants on LB XGal indicator medium as well as on MacConkey lactose medium.
Shuttle expression vector allowing visual detection of recombinant molecules in a broad host range.
Constructed by insertion of a 1.8 kb PstI fragment from pRO1614 into the NarI site of pUC19.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Karkhoff-Schweizer RR, Schweizer HP. Utilization of a mini-Dlac transposable element to create an alpha-complementation and regulated expression system for cloning in Pseudomonas aeruginosa. Gene 140: 7-15, 1994. PubMed: 8125342

Schweizer HP. Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 97: 109-112, 1991. PubMed: 1899844

Shipped frozen
Shipping Information

 Freeze dried Escherichia coli DH5alphaF’ containing the plasmid.

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