pCRE1 (ATCC® 77368)

Organism: bacteriophage P1  /  Clone Type: Clone  /  Depositors: IN Maruyama

Permits and Restrictions

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Designations pCRE1
Species bacteriophage P1
Depositors IN Maruyama
Applications
produces protein recombinase, site-specific
Vector
Construct size (kb): 5.960000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: ScaI
Vector end: ScaI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: bacteriophage P1
Gene symbol: cre
Gene name: recombinase, site-specific
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: PvuII
Insert end: PvuII
Insert size (kb): 1.6
Cross references:
Insert lengths(kb): 1.600000023841858
Gene product: recombinase, site-specific [cre]
Target Gene: recombinase, site-specific
Insert Size (kb) 1.600
Media Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Related Products
component of:ATCC 77370
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.05; XmnI--3.1, 2.4, 0.45; HindIII--5.2, 0.75; BamHI--4.4, 1.55; EcoRI/PstI--3.5, 1.4, 0.7, 0.35.
Expresses the Cre recombinase from a lacZ promoter. Insert-containing lambdaMGU2 (ATCC 77367) cannot grow productively in this RecA- strain, so excised phagemid are recovered as ampR survivors of phage infection.
References

Maruyama IN, Brenner S. A selective lambda phage cloning vector with automatic excision of the insert in a plasmid. Gene 120: 135-141, 1992. PubMed: 1327972

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