pCRE1 (ATCC® 77368)

Organism: bacteriophage P1  /  Clone Type: Clone  /  Depositors: IN Maruyama

Permits and Restrictions

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Designations pCRE1
Species bacteriophage P1
Depositors IN Maruyama
Applications
produces protein recombinase, site-specific
Vector
Construct size (kb): 5.960000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: ScaI
Vector end: ScaI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: bacteriophage P1
Gene symbol: cre
Gene name: recombinase, site-specific
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: PvuII
Insert end: PvuII
Insert size (kb): 1.6
Cross references:
Insert lengths(kb): 1.600000023841858
Gene product: recombinase, site-specific [cre]
Target Gene: recombinase, site-specific
Insert Size (kb) 1.600
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Related Products
component of:ATCC 77370
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.05; XmnI--3.1, 2.4, 0.45; HindIII--5.2, 0.75; BamHI--4.4, 1.55; EcoRI/PstI--3.5, 1.4, 0.7, 0.35.
Expresses the Cre recombinase from a lacZ promoter. Insert-containing lambdaMGU2 (ATCC 77367) cannot grow productively in this RecA- strain, so excised phagemid are recovered as ampR survivors of phage infection.
References

Maruyama IN, Brenner S. A selective lambda phage cloning vector with automatic excision of the insert in a plasmid. Gene 120: 135-141, 1992. PubMed: 1327972

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