pUR18 (ATCC® 77297)

Applications: expression vectorshuttle vectorvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: NC Barbet, AM Carr

Permits and Restrictions

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Designations pUR18
Depositors NC Barbet, AM Carr
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5000000000000000
Vector: pUR18 (plasmid)
Promoters: Promoter lac
Construction: pUC18, pON163
Construct size (kb): 5.5
Features: insert detection: lacZ'
marker(s): ampR
marker(s): ura4+
promoter: lac
replicon: ars1
replicon: pMB1
expression vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5.
Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation.
Constructed by inserting a 1.7 kb ClaI fragment containing ura4 and a 1.2 kb ClaI fragment containing ars1 into the NdeI site modified with a ClaI linker.
The order of the major features in this plasmid is: ars1 - ura4 - HindIII/MCS/EcoRI/lacZ' - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485

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Shipped frozen
Shipping Information Freeze dried E. coli containing the plasmid
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