pJAK12 [JK386] (ATCC® 77287)

Applications: expression vectorshuttle vector  /  Depositors: JA Kornacki

Permits and Restrictions

View Permits

Designations pJAK12 [JK386]
Depositors JA Kornacki
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli SCS1
Vector Information
Size (kb): 10.8000001907348600
DESCRIPTION OF VECTOR:
Intact vector size: 10.800
Type of vector: plasmid
Cloning sites: EcoRI SacI Ecl136II KpnI SmaI XmaI BamHI XbaI SalI PstI
HindIII
Polylinker sites: EcoRI SacI Ecl136II KpnI XmaI SmaI BamHI XbaI AccI SalI
HincII PstI SphI HindIII
Construction: pMMB67EH (ATCC 37622), R100.1
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
promoter: tac, ->
MCS: EcoRI...HindIII, ->
transcription terminator: rrnB
restriction site: SphI
marker(s): strR (spcR)
replicon: oriV
replicon: oriT
replicon: RSF1010
repressor gene: lacIq
Vector: pJAK12 (plasmid)
Promoters: Promoter tac
Construction: pMMB67EH (ATCC 37622), R100.1
Marker(s):strR (spcR)
Construct size (kb): 10.80000019073486
Features: marker(s): strR (spcR)
promoter: tac
replicon: RSF1010
replicon: oriT
replicon: oriV
repressor gene: lacIq
MCS: EcoRI...HindIII
restriction site: SphI
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--10.8; HindIII--10.8; SphI--9.7, 1.1.
A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria.
Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids.
Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria.
Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation.
Media ATCC® Medium 1445: Nutrient agar or broth with 20 mcg/ml streptomycin
Growth Conditions
Temperature: 37.0°C
References

Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457

Jon A Kornacki, personal communication

Related Products
component of:ATCC 87055
Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map