Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 4.3000001907348630
Vector: pNKY48 (plasmid)
Construction: pGC-1, pNKY2029
Construct size (kb): 4.300000190734863
Features: marker(s): ampR, URA3
replicon: pMB1, M13
YI-type (integrating) shuttle vector
contains easily purifiable cassette(s) for construction
contains sequence histidinol dehydrogenase
contains sequence uridine monophosphate synthetase UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Restriction digests of the clone give the following sizes (kb): PstI--4.4; BglII--4.4; HindIII--3.1, 1.3; EcoRI--4.4; XbaI--4.4.
Contains a HIS4C/URA3 fusion cassette that can be used to make fusions to other proteins of interest. Also includes a M13 sequencing primer approximately 300 bp from the HIS4C/URA3 fusion junction.
Encodes 93 amino acids of HIS4C and the complete 267 amino acids of URA3.
Can be used for promoter/enhancer trap screens.
Constructed by inserting a 1.4 kb HindIII fragment from pNKY2029 containing the HIS4/URA3 fusion into pNKY47, which is a derivative of pGC-1 with the original HindIII site destroyed and a new one created at the BamHI site of the MCS.
The order of the major features in this plasmid is: M13 primer - ClaI - XhoI - SalI - XbaI (stop) - BamHI - HindIII - 90 aa HIS4C bridge/URA3 - BamHI - HindIII - BamHI - BglII - NcoI - EcoRI - M13 ori - pMB1 ori - ampR.
Alani E, Kleckner N. A new type of fusion analysis applicable to many organisms: protein fusions to the URA3 gene of yeast. Genetics 117: 5-12, 1987. PubMed: 3311876
Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151
Nancy Kleckner, personal communication