pRS303 (ATCC® 77138)

Applications: YI-type (integrating) shuttle vectorshuttle vectorvector containing primer sites useful for sequencingvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: P Hieter

Permits and Restrictions

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Designations pRS303
Depositors P Hieter
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 4.4530000686645510
DESCRIPTION OF VECTOR:
Intact vector size: 4.453
Type of vector: phagemid
Cloning sites: ApaI XhoI SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI EagI
NotI SacII SacI NaeI
Polylinker sites: KpnI ApaI XhoI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI
XbaI EagI NotI BstXI SacII SacI
Construction: pRSS56 [pBluescript KS+, pBS(+)]
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): HIS3, ->
replicon: f1
insert detection: lacZ', <-
promoter: T7, ->
MCS:
promoter: T3, <-
replicon: pMB1
marker(s): ampR, <-
Vector: pRS303 (phagemid)
Promoters: Promoter T7
Construction: pRSS56 [pBluescript KS+, pBS(+)]
Marker(s):ampR,HIS3
Construct size (kb): 4.453000068664551
Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: T3
promoter: T7
replicon: f1
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.2, 1.1, 0.24; PvuII--4.0, 0.48; XbaI--4.5; EcoRI--4.5.
One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene.
YI-type integrating shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide.
pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+).
A fragment (1.184 kb) containing the HIS3 gene was inserted into the NdeI site of pRSS56. All ends were blunted.
The following restriction sites in the multiple cloning site are no longer unique: KpnI HindIII PstI BstXI.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

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component of:ATCC 77189
Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 77138