Oncogen, BW Poor, Oncogen
Construct size (kb): 8.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pEMBL18+
Intact vector size: 3.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
replicon: pMB1, f1
DESCRIPTION OF INSERT COMPONENT:
Gene symbol: MFI2
Genomic copy number: unique
Gene name: antigen p97 (melanoma associated) identified by monoclonal
antibodies 133.2 and 96.5
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3 q28-q29
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: EcoRI
Insert size (kb): 4.4
Cross references: DNA Seq. Acc.: M12154
Insert lengths(kb): 4.400000095367432
Gene product: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 [MFI2]
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.8, 2.75, 1.5; BamHI--6.6, 1.4; SalI--8.0.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.3, 2.6; HindIII--2.7; PvuII--0.6, 1.6; SstI--0.4, 1.7, 2.3; SalI--1.3.
Two errors in the sequence of the insert have been reported. Because of a base change, the lysine coded for at nt 1351 becomes threonine and a missing cytosine at nt 1930 causes an early truncation.
Digestion with EcoRI and HindIII excises the insert in two fragments. The larger fragment contains the complete coding sequence, that extends approximately from nt 60 to nt 2300.
Brown JP, et al. Vaccines against melanoma. US Patent 5,141,742 dated Aug 25 1992
Brian W Poor, personal communication
Trish Amarante, personal communication