p97b (ATCC® 53403)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: Oncogen, BW Poor, Oncogen

Permits and Restrictions

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Designations p97b
GenBank Number

M12154

Species Homo sapiens, human
Depositors Oncogen, BW Poor, Oncogen
Vector
Construct size (kb): 8.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pEMBL18+
Intact vector size: 3.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: MFI2
Genomic copy number: unique
Gene name: antigen p97 (melanoma associated) identified by monoclonal
antibodies 133.2 and 96.5
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3 q28-q29
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: EcoRI
Insert size (kb): 4.4
Cross references: DNA Seq. Acc.: M12154
Insert lengths(kb): 4.400000095367432
Gene product: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 [MFI2]
Insert Size (kb) 4.400
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
transformed cell line:ATCC CRL-8985
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.8, 2.75, 1.5; BamHI--6.6, 1.4; SalI--8.0.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.3, 2.6; HindIII--2.7; PvuII--0.6, 1.6; SstI--0.4, 1.7, 2.3; SalI--1.3.
Two errors in the sequence of the insert have been reported. Because of a base change, the lysine coded for at nt 1351 becomes threonine and a missing cytosine at nt 1930 causes an early truncation.
Digestion with EcoRI and HindIII excises the insert in two fragments. The larger fragment contains the complete coding sequence, that extends approximately from nt 60 to nt 2300.
References

Brown JP, et al. Vaccines against melanoma. US Patent 5,141,742 dated Aug 25 1992

Brian W Poor, personal communication

Trish Amarante, personal communication

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