pGL2C (ATCC® 53367)

Applications: expression vector  /  Depositors: Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.

Permits and Restrictions

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Designations pGL2C
Depositors Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.
Biosafety Level 1
Host
Distribution host: Escherichia coli JM83; K-12 strain JM83
Vector Information
Size (kb): 6.5999999046325680
Vector: pGL2C (plasmid)
Promoters: Promoter glucoamylase
Construction: pUC12
Marker(s):ampR
Construct size (kb): 6.599999904632568
Features: marker(s): ampR
promoter: glucoamylase
replicon: pMB1
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.0; BglII--7.0, EcoRI--4.6, 1.8, 0.33.
One of 3 vectors (pGL2A ATCC 53365, pGL2B 53366, pGL2C ATCC 53367) differing in the length of a synthetic linker and thus providing alternate reading frames.
Constructed by inserting a HindIII/BglII fragment containing the glucoamylase gene into pUC12. A BssHII/SstI fragment (starting just 3' to the region coding the signal peptide) was then replaced with a synthetic linker having EcoRV and BglII sites.
Growth Conditions
Temperature: 37.0°C
References

Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet