pGL2A (53365)

Applications: expression vectorvector with sequences for protein export  /  Depositors: Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.

Permits and Restrictions

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Designations pGL2A
Depositors Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.
Biosafety Level 1
Distribution host: Escherichia coli JM83; K-12 strain JM83
Vector Information
Size (kb): 6.5999999046325680
Vector: pGL2A (plasmid)
Promoters: Promoter glucoamylase
Construction: pUC12
Construct size (kb): 6.599999904632568
Features: marker(s): ampR
promoter: glucoamylase
replicon: pMB1
expression vector
vector with sequences for protein export
Restriction digests of the clone give the following sizes (kb): EcoRI--4.6, 1.9; BamHI--6.6; HindIII--6.6.
One of 3 vectors (pGL2A ATCC 53365, pGL2B 53366, pGL2C ATCC 53367) differing in the length of a synthetic linker and thus providing alternate reading frames.
Constructed by inserting a HindIII/BglII fragment containing the glucoamylase gene into pUC12. A BssHII/SstI fragment (starting just 3' to the region coding the signal peptide) was then replaced with a synthetic linker having EcoRV and BglII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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