Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47025)

Strain Designations: BNN102 [C600hflA150, Y1073]  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations BNN102 [C600hflA150, Y1073]
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Preceptrol® no
Genotype F- hflA150::Tn10 supE44 lacY1 thr-1 leuB6 mcrA1 mcrB thi-1 fhuA21 e14- hsdR lambda-
Type Strain no
Comments
Positive selection host for recombinant lambdagt10 libraries. By suppressing plaque formation of cI+ phage, it plates only recombinant phage.
Medium Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype F- hflA150::Tn10 supE44 lacY1 thr-1 leuB6 mcrA1 mcrB thi-1 fhuA21 e14- hsdR lambda-
Name of Depositor R Young, RW Davis
References

Huynh TV, et alConstructing and screening cDNA libraries in lambdagt10 and lambdagt11In: Huynh TV, et alDNA cloning techniques: a practical approach1OxfordIRL Presspp. 49-78, 1985

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation