Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47020)

Strain Designations: WM6  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations WM6
Application
protease-deficient transformation host
transformation host
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli

Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype F- supC(Ts) lac(Am) mal trp(Am) rpsL htpR165 pho(Am) lambda-
Type Strain no
Comments
The depositor indicated that this strain was derived from CAG-456. The genotype is not complete because the nature of the reversion is not known.
This strain has reduced proteolysis of foreign proteins. It does not require heat induction to reduce proteolysis and has the potential to accumulate foreign protein over a longer period of time.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C
Genotype F- supC(Ts) lac(Am) mal trp(Am) rpsL htpR165 pho(Am) lambda-
Name of Depositor W Mandecki
References

Mandecki W, et al. High-level expression of a gene encoding the human complement facator C5a in Escherichia coli. Gene 43: 131-138, 1986. PubMed: 3530879

Baker TA, et al. A gene regulating the heat shock response in Escherichia coli also affects proteolysis. Proc. Natl. Acad. Sci. USA 81: 6779-6783, 1984. PubMed: 6387713