AM6 [EC-AM6, pAM6] (ATCC® 39630)

Organism: Hepatitis B virus, Hepatitis B virus  /  Clone Type: Clone  /  Depositors: Scripps Clinic and Research Foundation, AM Moriarity, Scripps Clinic and Research Foundation

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Designations AM6 [EC-AM6, pAM6]
Species Hepatitis B virus, Hepatitis B virus
Depositors Scripps Clinic and Research Foundation, AM Moriarity, Scripps Clinic and Research Foundation
Vector
Construct size (kb): 7.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: hepatitis B virus
Gene name: genome, full-length
Contains complete coding sequence?: U
Strain: serotype adw
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 3.2
Cross references:
Insert lengths(kb): 3.20
Insert Size (kb) 3.200
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Shipping Information Distributed: frozen
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.5, 1.85, 1.35; HindIII--7.5; EcoRI--7.5; BglII/PstI--5.2, 1.35, 0.96.
A BamHI digest cuts the insert into fragments of 1.85 kb and 1.35 kb. The 1.35 kb fragment contains coding sequences for HBsAg and most of region P. The 1.85 kb fragment encodes HBcAg and most of the X gene product.
AM6 is available as ATCC 39630, 40101, 45020 and 45020D.
Molecular clone of linearized hepatitis genome
The 1350 bp BamHI fragment encodes both group and subtype-specific determinants of HBsAg.
The virus was isolated from plasma (subtype adw) from an HBsAg-positive donor. DNA was extracted from purified Dane particles.
The complete viral genome was linearized with BamHI and inserted into pBR322.
Classification Hepadnaviridae, Orthohepadnavirus
References

Moriarty AM, et al. Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector. Proc. Natl. Acad. Sci. USA 78: 2606-2610, 1981. PubMed: 6264484

Moriarty AM. SV40 expression vector containing HBxAg as an expression marker. US Patent 4,942,125 dated Jul 17 1990

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