PMLBKTK [143B PML BK TK] (ATCC® 39369)

Organism: BK polyomavirus, BK Virus  /  Clone Type: Clone  /  Depositors: Wistar Institute

Permits and Restrictions

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Designations PMLBKTK [143B PML BK TK]
Species BK polyomavirus, BK Virus
Depositors Wistar Institute
Vector
Construct size (kb): 12.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: PML
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: BK virus
Gene name: genome, partial
Contains complete coding sequence?: U
Genome: herpes simplex virus
Gene symbol: tk
Gene name: thymidine kinase
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb):
Cross references:
Gene product: genome, partial [tk]
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
transformed cell line:ATCC CRL-8304
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--8.4, 4.0; EcoRI--6.4, 3.4, 2.6; PstI--6.4, 2.4 (doublet), 0.9.
Also available transfected into 143B (CRL-8303) as 143B PML BK TK (CRL-8304).
The viral inserts contain deletions to render the viral DNAs harmless.
Classification Polyomaviridae, Polyomavirus
References

Robert Ricciardi, personal communication

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