PMLBKTK [143B PML BK TK] (ATCC® 39369)

Organism: BK polyomavirus, BK Virus  /  Clone Type: Clone  /  Depositors: Wistar Institute

Permits and Restrictions

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Designations PMLBKTK [143B PML BK TK]
Species BK polyomavirus, BK Virus
Depositors Wistar Institute
Vector
Construct size (kb): 12.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: PML
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: BK virus
Gene name: genome, partial
Contains complete coding sequence?: U
Genome: herpes simplex virus
Gene symbol: tk
Gene name: thymidine kinase
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb):
Cross references:
Gene product: genome, partial [tk]
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
transformed cell line:ATCC CRL-8304
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--8.4, 4.0; EcoRI--6.4, 3.4, 2.6; PstI--6.4, 2.4 (doublet), 0.9.
Also available transfected into 143B (CRL-8303) as 143B PML BK TK (CRL-8304).
The viral inserts contain deletions to render the viral DNAs harmless.
Classification Polyomaviridae, Polyomavirus
References

Robert Ricciardi, personal communication