pWE15 (ATCC® 37503)

Applications: contains easily purifiable cassette(s) for constructionexpression vectorintegrating vectorshuttle vectorvector for constructing genomic librariesvector permitting RNA synthesis in vitrovector permitting positive selection for integration  /  Depositors: GA Evans

Permits and Restrictions

View Permits

Designations pWE15
Depositors GA Evans
Biosafety Level 1
Vector Information
Size (kb): 8.1999998092651370
Vector: pWE15 (cosmid)
Promoters: Promoter T3
Marker(s):G418R,ampR,kanR
Construct size (kb): 8.199999809265137
Features: marker(s): ampR, kanR, G418R
promoter: T3, T7
replicon: pMB1
Applications
contains easily purifiable cassette(s) for construction
expression vector
integrating vector
shuttle vector
vector for constructing genomic libraries
vector permitting RNA synthesis in vitro
vector permitting positive selection for integration
Comments
pWE15 (ATCC 37503) and pWE16 (ATCC 37524) differ only in the markers selectable in vertebrate cells.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Cosmid vector for rapid genomic walking, restriction mapping and gene transfer, with selectable markers in either Escherichia coli or mammalian cell lines. Contains promoters T3 and T7 flanking the BamHI site.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Wahl GM, et al. Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer. Proc. Natl. Acad. Sci. USA 84: 2160-2164, 1987. PubMed: 3031668

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet