pAV2 (ATCC® 37216)

Applications: contains sequence human adeno-associated virus type 2expression vector  /  Depositors: CA Laughlin

Permits and Restrictions

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Designations pAV2
Depositors CA Laughlin
Biosafety Level 1
Vector Information
Size (kb): 8.6999998092651370
Vector: pAV2 (plasmid)
Promoters: Promoter P19
Construction: pA11P.Xba, AAV genome
Marker(s):ampR
Construct size (kb): 8.699999809265137
Features: marker(s): ampR
promoter: viral P5, P19, P40
replicon: pMB1, AAV
terminator: AAV polyadenylation
Applications
contains sequence human adeno-associated virus type 2
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BglII--4.7, 3.7; EcoRI--6.6, 1.8.
pAV1 (ATCC 37215) and pAV2 (ATCC 37216) both contain the complete AAV2 genome, but in opposite orientations. The pAV2 vector has fewer polylinker sites than that of pAV1 (HindIII, ClaI, and one EcoRI site have been deleted).
Contains the complete viral genome for use in site-specific mutagenesis studies. Transfection into human 293 cells with an Ad helper virus results in infectious AAV virus particles.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Laughlin CA, et al. Cloning of infectious adeno-associated virus genomes in bacterial plasmids. Gene 23: 65-73, 1983. PubMed: 6352411

Wondisford FE, et al. Cloning of the human thyrotropin beta-subunit gene and transient expression of biologically active human thyrotropin after gene transfection. Mol. Endocrinol. 2: 32-39, 1988. PubMed: 3398841

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