pRK2013 (ATCC® 37159™)

Applications: mobilization helper plasmid  /  Depositors: EW Nester

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations pRK2013
Depositors EW Nester
Biosafety Level 1
Vector Information
Size (kb): 48.0
Vector: pRK2013 (plasmid)
Construction: pPRK212.2, pDF1
Marker(s): kanR
Construct size (kb): 48.0
Features:
marker(s): kanR
replicon: RK2
Applications
mobilization helper plasmid
Comments
This is a helper plasmid for mobilization of non-self-transmissible plasmids.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37°C
References

Knauf VC, Nester EW. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium ti plasmid. Plasmid 8: 45-54, 1982. PubMed: 6292969

Figurski DH, Helinski DR. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76: 1648-1652, 1979. PubMed: 377280

Shipped FZ
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet
Product Sheet
Product Sheet