pMOB48 (ATCC® 37107)

Applications: expression vector  /  Depositors: D Vapnek

Designations pMOB48
Depositors D Vapnek
Biosafety Level 1
Vector Information
Size (kb): 9.5000000000000000
Vector: pMOB48 (plasmid)
Promoters: Promoter lacUV5
Construction: R1, M13mp7
Marker(s):cmlR
Construct size (kb): 9.5
Features: insert detection: lacZ'
marker(s): cmlR
promoter: lacUV5
replicon: R1(ts)
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.5; EcoRI--7.2, 2.3; HindIII--8.5, 1.0.
An expression vector with copy number amplifiable by heat induction without protein synthesis inhibition. Can be used for lacIq-controlled expression of proteins.
Media Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 30.0°C
References

Bittner M, Vapnek D. Versatile cloning vectors derived from the runaway-replication plasmid pKN402. Gene 15: 319-329, 1981. PubMed: 6277736

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet