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Clostridium thermocellum Viljoen et al. (ATCC^® 27405^™)

Strain Designations: VPI 7372 [157] / Type Strain: yes / Biosafety Level: 1

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Strain Designations	VPI 7372 [157]
Application	Produces endoglucanase Produces restriction endonuclease CthI Produces restriction endonuclease CthII Biotechnology
Type Strain	yes
Biosafety Level	1
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C

Genome Sequenced Strain	Clostridium thermocellum ATCC 27405, complete genome.
Type Strain	yes
Comments	Genome sequenced strain

Medium	ATCC^® Medium 1191: Clostridium thermocellum medium (ATCC medium 1190) with 18.75 g filter paper substituted for the glucose ATCC^® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions	Temperature: 55°C Atmosphere: Anaerobic gas mixture

Name of Depositor	LD Smith
Chain of Custody	ATCC <-- LD Smith <-- J Alexander <-- R McBee, J Alexander <-- R McBee
References	Johnson EA. Saccharification of complex cellulosic substrates by the cellulase system from Clostridium thermocellum. Appl. Environ. Microbiol. 43: 1125-1132, 1982. McBee RH. The characteristics of Clostridium thermocellum. J. Bacteriol. 67: 505-506, 1954. PubMed: 13152068 Korean Biochem. J. 23: 418-421, 1990. Korean J. Appl. Microbiol. Bioeng. 15: 352-355, 1987. Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980.
Cross References	Nucleotide (GenBank) : M67438 Clostridium thermocellum xylanase (xynX) gene, complete cds. Nucleotide (GenBank) : AF148212 Clostridium thermocellum hydrogenase-1 (hydA) gene, partial cds. Nucleotide (GenBank) : CP000568 Clostridium thermocellum ATCC 27405, complete genome.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS