Cryptococcus neoformans var. grubii (ATCC® 208821™)

Strain Designations: H99 [H99JP, NYSD 1649]  /  Product Format: frozen

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Deposited As Cryptococcus neoformans (Sanfelice) Vuillemin, anamorph
Strain Designations H99 [H99JP, NYSD 1649]
Application
Biomedical Research and Development Material
Emerging infectious disease research
Genome sequencing strain (Broad Institute, USA).
RefHeitman J, et al. The Cryptococcus neoformans genome sequencing project. Mycopathologia 148: 1-7, 1999.
Negative control in differential gene expression studies
Refdel Poeta M, et al. Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. Infect. Immun. 67: 1812-1820, 1999. PubMed: 10085022
produces laccase
RefWilliamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929
Biosafety Level 2
Product Format frozen
Type Strain yes (type strain of Cryptococcus neoformans var. grubii)
Antigenic Properties serotype A RefWilliamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929, RefFranzot SP, et al. Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates. J. Clin. Microbiol. 37: 838-840, 1999. PubMed: 9986871
Genome Sequenced Strain

Yes

Mating Type MATalpha (Peter R Williamson, personal communication)
Comments
Multigene phylogeny and phenotypic characterization.
RefFindley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot. Cell 8: 353-361, 2009.
Facultative intracellular pathogen
RefFeldmesser M, et al. Cryptococcus neoformans is a facultative intracellular pathogen in murine pulmonary infection. Infect. Immun. 68: 4225-4237, 2000. PubMed: 10858240
Medium ATCC® Medium 200: YM agar or YM broth
Growth Conditions
Temperature: 30°C
Atmosphere: Typical aerobic
Sequenced Data
Not available.
Name of Depositor PR Williamson
Special Collection NCRR Contract
Chain of Custody
ATCC <-- PR Williamson <-- JR Perfect
Isolation
Patient with Hodgkin's disease, New York
RefFranzot SP, et al. Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates. J. Clin. Microbiol. 37: 838-840, 1999. PubMed: 9986871
Cross References

Nucleotide (GenBank) : AF254790 Filobasidiella neoformans var. neoformans vacuolar (H+)-ATPase

Nucleotide (GenBank) : AF098972 phosphatidylinositol 3-kinase TOR1 (TOR1), complete coding sequence

Nucleotide (GenBank) : AF097888 macrolide-binding protein FKBP12 (FRR1) gene, complete coding sequence

Nucleotide (GenBank) : AF097889 macrolide-binding protein FKBP12 (FRR1) mRNA, complete coding sequence

Nucleotide (GenBank) : AACO02000000 Cryptococcus neoformans var. grubii H99, whole genome shotgun sequencing project.

References

Williamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929

Feldmesser M, et al. Cryptococcus neoformans is a facultative intracellular pathogen in murine pulmonary infection. Infect. Immun. 68: 4225-4237, 2000. PubMed: 10858240

del Poeta M, et al. Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. Infect. Immun. 67: 1812-1820, 1999. PubMed: 10085022

Franzot SP, et al. Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates. J. Clin. Microbiol. 37: 838-840, 1999. PubMed: 9986871

Cruz MC, et al. Rapamycin antifungal action is mediated via conserved complexes with FKBP12 and TOR kinase homologs in Cryptococcus neoformans. Mol. Cell. Biol. 19: 4101-4112, 1999. PubMed: 10330150

Heitman J, et al. The Cryptococcus neoformans genome sequencing project. Mycopathologia 148: 1-7, 1999.

type strain of Cryptococcus neoformans var. grubii

Peter R Williamson, personal communication

Findley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot. Cell 8: 353-361, 2009.

Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009.

Ding C, et al. The copper regulon of the human fungal pathogen Cryptococcus neoformans H99. Mol. Microbiol. 81: 1560-1576, 2011. PubMed: 21819456

Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334

type strain of Cryptococcus neoformans var. grubii

Williamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929

Franzot SP, et al. Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates. J. Clin. Microbiol. 37: 838-840, 1999. PubMed: 9986871

Peter R Williamson, personal communication

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS