Unlock precision in assay development with ATCC’s ISO 9001–guided genomic DNA (gDNA) from authenticated human cell lines. These high-quality nucleic acids help researchers validate workflows, benchmark platforms, and ensure consistent performance across applications. Whether you're working on NGS validation, residual DNA quantification, or instrument comparison, ATCC gDNA provides trusted, ready-to-use positive controls and reference standards to support every step—from sample to sequence.

• Streamline assay development with ready-to-use nucleic acids from clinically relevant cell lines.
• Eliminate the need for cell culturing and extraction, saving time and resources.
• Support inclusivity and exclusivity testing with stabilized nucleic acid preparations.
• Establish limits of detection using verified whole-genome materials.
• Validate and compare diagnostic test methods with consistent, high-quality controls.

Quantitative nucleic acids  Qualitative nucleic acids  Explore the portfolio

ATCC’s quantitative cell line genomic DNA offers a reliable, sustainable alternative to patient tissue-derived controls for oncology molecular assays. Extracted from validated samples and quantified by next-generation sequencing and digital PCR, ATCC quantitative DNA are ready-to-use controls compatible with lab-developed and commercial assays. Designed for rigorous validation, these nucleic acids support cross-platform comparisons and algorithm benchmarking.

Key Features:

• Contains clinically relevant biomarkers
• Highly quantitated (3 μg gDNA/vial) 
• Supplied with known mutation allelic frequency
• Includes absolute gene copies per nanogram of DNA 
• Provided with relative gene copy number data
• Safe to use in BSL-1 facilities
• Compatible with lab-developed and commercial assays
• Evaluated to confirm identity, purity, and functional activity
  
   

Applications:

• Develop molecular-based assays with reliable positive controls
• Validate instruments and workflows across platforms
• Generate standard curves for quantitative analysis
• Compare algorithms and benchmark assay performance
• Monitor assay-to-assay and lot-to-lot variation
• Analyze copy number variants (CNV) and loss of heterozygosity (LOH)

ATCC's qualitative nucleic acids are high-quality, ready-to-use DNA preparations derived from authenticated human cell lines. These materials are designed to support molecular assay development, validation, and diagnostic research by providing consistent and reliable reference standards. These controls are primarily used to verify the presence or absence of genetic material, assess assay specificity, and ensure inclusivity/exclusivity in test systems.

Key Features:

• Derived from authenticated source materials
• Provided in a stabilized and ready-to-use format
• Safe to use in BSL-1 facilities
• Verified for integrity, purity, and authenticity
• Compatible with lab-developed and commercial assays
• Evaluated to confirm functional activity

Applications:

• Inclusivity/exclusivity testing
• Benchmarking and comparative analysis 
• Assay validation and method comparison
• Comparative analysis of test methods
• Cancer genome research
• Molecular diagnostics and assay development