Escherichia coli (Migula) Castellani and Chalmers (ATCC® 43895)

Strain Designations: CDC EDL 933  /  Type Strain: no  /  Biosafety Level: 2

Classification Enterobacteriaceae, Escherichia
Strain Designations CDC EDL 933
Application
Media testing
Produces Shiga-like toxin I
Produces Shiga-like toxin II
Quality control strain
Quality control strain for Difco products
Isolation
Raw hamburger meat implicated in hemorrhagic colitis outbreak
Type Strain no
Biosafety Level 2
Antigenic Properties Serotype O157:H7
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antigenic Properties Serotype O157:H7
Medium Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor CDC
Isolation
Raw hamburger meat implicated in hemorrhagic colitis outbreak
References

Strockbine NA, et al. Two toxin-converting phages from Escherichia coli O157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infect. Immun. 53: 135-140, 1986. PubMed: 3522426

Wells JG, et al. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J. Clin. Microbiol. 18: 512-520, 1983. PubMed: 6355145

Marques LR, et al. Production of Shiga-like toxin by Escherichia coli. J. Infect. Dis. 154: 338-341, 1986. PubMed: 3522760

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

Cross References

Nucleotide (GenBank) : X96953 E. coli espB gene.

Nucleotide (GenBank) : AX110209 Sequence 942 from Patent WO0123604.

Nucleotide (GenBank) : AX110240 Sequence 973 from Patent WO0123604.

Nucleotide (GenBank) : L31880 Escherichia coli ribosomal RNA sequence.