Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv) (ATCC® VR-1450)

Classification: Retrovirus, Mammalian Type C Retrovirus, Mammalian Group, Amphotropic  /  Product Format: frozen

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Classification Retrovirus, Mammalian Type C Retrovirus, Mammalian Group, Amphotropic
Agent Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv)
Strain 4070A envelope strain
Applications
Analytical methodologies
Pharmaceutical and Personal Care
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Virus was harvested from tk(-) NIH3T3 cells transfected with molecular clone of MLV (VR-1448)
Product Format frozen
Storage Conditions -70°C or colder
Comments
This virus produces plaques on PG-4 (feline S+L-) cells (ATCC CRL-2032), and foci of rounded cells on MiCL1 (Mink S+L-) cells (ATCC CCL-64.1). This holding is prepared by growing ATCC VR-1448 and harvesting the supernate.
At the time of deposit, cell-free preparations of the Mo/A-MuLV strain were considered as suitable reference standards in assays to detect replication competent murine retrovirus--select lots have had titer confirmed by workers outside the ATCC.
amphotropic
Effect on Host
No, in vitro effects: Cytopathic effects in NIH3T3 cells
No CPE in NIH3T3 cells
Recommended Host
NIH/3T3 (tk-) (ATCC CRL-1658)

Recommended Host: tk(-) NIH3T3 (ATCC® CRL-1658™)

many mammalian cell lines (mouse, rat, human)
Growth Conditions
Duration: 5-7 days at 37.0°C in NIH/3T3 (tk-) (ATCC CRL-1658)
Cryopreservation
Storage temperature: -70C
Effect on Host
No, in vitro effects: Cytopathic effects in NIH3T3 cells
No CPE in NIH3T3 cells
Name of Depositor CA Wilson
Special Collection NCRR Contract
Source
Virus was harvested from tk(-) NIH3T3 cells transfected with molecular clone of MLV (VR-1448)
References

Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952

General Chapters: <1046> Cell and Gene Therapy Products - Analytical Methodologies. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

Cross References

Nucleotide (GenBank) : AF010170 Plasmid pAMS with hybrid amphotropic/Moloney murine leukemia virus, complete sequence.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation