Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv) (ATCC® VR-1448)

Classification: Retrovirus, Mammalian Type C Retrovirus, Mammalian Group, Amphotropic  /  Product Format: frozen

Classification Retrovirus, Mammalian Type C Retrovirus, Mammalian Group, Amphotropic
Agent Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv)
Strain 4070A envelope strain
Applications
Analytical methodologies
Pharmaceutical and Personal Care
Biosafety Level 2
Isolation
Transfection of tk(-)NIH3T3 cells with molecular clone of MLV derived by A. Dusty Miller
Product Format frozen
Storage Conditions Vapor phase of liquid nitrogen
Comments
This virus produces foci of round cells on MiCL1(Mink S+L-) cell line, and plaques on PG-4 (feline S+L- cell line). This holding consists of persistently infected tk(-)NIH3T3 producer cells that shed the Mo/A-MLV virus.
amphotropic
Effect on Host
No CPE in NIH3T3 cells
Recommended Host
NIH/3T3 (tk-) (ATCC CRL-1658)

Recommended Host: This virus is continuously produced by the cell line in which it is shipped.  No additional host is required, however, if desired the virus may be used to infect other cells, which are listed as alternate hosts.

most mammalian cell lines (except certain bovine and hamster cells))
Cryopreservation
Storage temperature: -70C
Effect on Host
No CPE in NIH3T3 cells
Name of Depositor CA Wilson
Source
Transfection of tk(-)NIH3T3 cells with molecular clone of MLV derived by A. Dusty Miller
References

Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952

General Chapters: <1046> Cell and Gene Therapy Products - Analytical Methodologies. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011