Acholeplasma laidlawii (Sabin) Edward and Freundt (ATCC® qCRM-23206D)

Strain Designations: Genomic DNA from Acholeplasma laidlawii strain PG8 [NCTC 10116, PG8; A]  /  Type Strain: yes  /  Biosafety Level: 1

Strain Designations Genomic DNA from Acholeplasma laidlawii strain PG8 [NCTC 10116, PG8; A]
Application Quantitative external control produced under an ISO Guide 34 accredited process for use in testing and calibration in ISO 17025 accredited laboratories, inclusivity/exclusivity testing, establishing limits of detection, verification or comparison of test methods, and other molecular applications.
Type Strain yes
Biosafety Level 1
Product Format frozen
Specification range: 1x106 - 1x107 genome copies/µL
Storage Conditions -70°C or colder
Intended Use
Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

ACLASS Accredited Reference Material Producer AR-1384

Preceptrol® no
Genome Sequenced Strain

Acholeplasma laidlawii PG-8A, complete genome.

Type Strain yes
Comments Genome copy number is based on the quantification of the 16S rRNA gene from nine averaged samples. Acholeplasma laidlawii (ATCC 23206) has two copies of the 16S rRNA gene per cell.

The reported genome copy number may vary depending on quantitation method used.

The limits of detection for this nucleic acid preparation will depend on the set of primers used. Primer efficiency should be determined empirically.

This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications.
*The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.

Droplet Digital™ PCR is a trademark of Bio-Rad Laboratories, Inc.
Name of Depositor ATCC
Cross References

Nucleotide (GenBank) : X15508 Acholeplasma laidlawii gene for tRNA-Trp.

Nucleotide (GenBank) : U14905 Acholeplasma laidlawii PG8 ATCC 23206 16S ribosomal RNA gene, partial sequence.

Nucleotide (GenBank) : AF294996 Acholeplasma laidlawii 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence.

Nucleotide (GenBank) : CP000896 Acholeplasma laidlawii PG-8A, complete genome.