Human poliovirus 1 (ATCC® VR-59)

Classification: Picornaviridae, Enterovirus, Enterovirus C  /  Product Format: freeze-dried

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Classification Picornaviridae, Enterovirus, Enterovirus C
Agent Human poliovirus 1
Strain LSa
Applications
testing
Biosafety Level 2
Isolation
LS-a virus is a variant or mutant isolated from the Mahoney strain. (The Mahoney strain was isolated by T. Francis in 1941 from a pool of 3 stools from asymptomatic cases of poliomyelitis in Cleveland.)
Product Format freeze-dried
Storage Conditions -70°C or colder
Comments
[The BMBL notes that all laboratory personnel working directly with the agent must have documented polio vaccination or demonstrated serologic evidence of immunity to all three poliovirus types. (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4)].
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, rounded and sloughing cells in culture.
Yes, in vivo effects: paralysis in mouse
Recommended Host
mouse, i. s. inoculation; LLC-MK2 cells (ATCC CCL-7)

Production Host: LLC-MK2 (ATCC® CCL-7)

mouse; monkey, i. s. inoculation; monkey kidney cells
Growth Conditions
Duration: 2-10 days
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, rounded and sloughing cells in culture.
Yes, in vivo effects: paralysis in mouse
Name of Depositor M Schaeffer
Source
LS-a virus is a variant or mutant isolated from the Mahoney strain. (The Mahoney strain was isolated by T. Francis in 1941 from a pool of 3 stools from asymptomatic cases of poliomyelitis in Cleveland.)
References

Li CP, Schaeffer M. Adaptation of type I poliomyelitis virus to mice. Proc. Soc. Exp. Biol. Med. 82: 477-481, 1953. PubMed: 13047436

Li CP, et al. Experimentally produced variants of poliomyelitis virus combining in vivo and in vitro techniques. Ann. N.Y. Acad. Sci. 61: 902-910, 1955. PubMed: 13340599

Water supply -- Domestic type water treatment appliances -- Performance requirements. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4348:1995.

Cross References

Nucleotide (GenBank) : AJ295187 Human poliovirus 1 genomic RNA for partial polyprotein gene, isolate VR-59, ATCC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation