Kluyveromyces marxianus (EC Hansen) van der Walt (ATCC® 8554)

Alternate State: Candida kefyr (Beijerinck) van Uden et Buckley, anamorph  /  Strain Designations: [15, type E, ATCC 34439, CBS 5795, CCRC 21480, DSM 5418, NCYC 1426, NRRL Y-1109, UCD 55-61, VTTC-81112]  /  Product Format: freeze-dried

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Deposited As Kluyveromyces fragilis (Jorgensen) van der Walt, teleomorph
Strain Designations [15, type E, ATCC 34439, CBS 5795, CCRC 21480, DSM 5418, NCYC 1426, NRRL Y-1109, UCD 55-61, VTTC-81112]
Alternate State Candida kefyr (Beijerinck) van Uden et Buckley, anamorph
Application
assay of trichothecenes
bacterial resistance testing adhesives
degrades glucose D-glucose
degrades whey permeate
ferments pentoses
fungus resistance testing adhesives
produces acetoin dehydrogenase diacetyl reductase
produces beta-galactosidase beta-D-galactosidase
produces biomass
produces butanediol dehydrogenase acetoin reductase
produces ethyl alcohol ethanol
produces glycerol glycerin, glycerine
produces lactase
produces single-cell protein SCP
produces biomass on whey permeate of cheddar cheese
Biosafety Level 1
Product Format freeze-dried
Type Strain no
Preceptrol® no
Morphology

After 3 days cells are globose, ellipsoidal, to cylindrical in single, pairs, or short chains.  Growth is butyrous, glossy, cream-colored to brown.

Medium Medium 200: YM agar or YM broth
Growth Conditions
Temperature: 24.0°C
Sequenced Data
No DNA sequencing was performed in house on this product.
Morphology

After 3 days cells are globose, ellipsoidal, to cylindrical in single, pairs, or short chains.  Growth is butyrous, glossy, cream-colored to brown.

Name of Depositor Lederle Labs.
Chain of Custody
ATCC <<--Lederle Labs.<<--N.C. Laffer 15, type E
Isolation
Meadowgold creamery, USA
References

Crisan EV, Sorensen SG. Production and utilization of lactase. US Patent 4,007,283 dated Feb 8 1977

Schwarz JG, Hang YD. Study of acetoin reductase from Kluyveromyces marxianus. Lett. Appl. Microbiol. 16: 14-16, 1993.

Maleszka R, Schneider H. Fermentation of D-xylose, xylitol, and D-xylulose by yeasts. Can. J. Microbiol. 28: 360-363, 1982. PubMed: 6211222

Madhyastha MS, et al. Optimization of yeast bioassay for trichothecene mycotoxins. J. Food Prot. 57: 490-495, 1994.

chen KC, et al. Search method for the optimal medium for the production of lactase by Kluyveromyces fragilis. Enzyme Microb. Technol. 14: 659-664, 1992.

ASTM International Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4783-01e1.

Mahoney RR, et al. Selection of strain, growth conditions, and extraction procedures for optimum production of lactase from Kluyveromyces fragilis. J. Dairy Sci. 58: 1620-1629, 1975. PubMed: 1194465

Mahoney RR. Number and nature of the sulphydryl groups of beta-galactosidase from Kluyveromyces fragilis. J. Food Biochem. 4: 189-199, 1980.

DeLaney RA, et al. Composition of Saccharomyces fragilis biomass grown on lactose permeate. J. Sci. Food Agric. 26: 1177-1186, 1975.

Inchaurrondo VA, et al. Yeast growth and þ-galactosidase production during aerobic batch cultures in lactose-limited synthetic medium. Process Biochem. 29: 47-54, 1994.

Schwarz JG, Hang YD. Kluyveromyces marxianus: a potential source of diacetyl reductase. World J. Microbiol. Biotechnol. 10: 385-387, 1994.

Rapin JD, et al. Glycerol production by yeast fermentation of whey permeate. Enzyme Microb. Technol. 16: 143-150, 1994.

Freer SN, Detroy RW. Characterization of cellobiose fermentations to ethanol by yeasts. Biotechnol. Bioeng. 25: 541-557, 1983.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation