|
Permits
|
These permits may be required for shipping this product:
-
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
|
|
Species
|
Escherichia coli (Migula) Castellani and Chalmers
|
|
Applications
|
detection of LT toxin produces protein heat-labile enterotoxin
|
|
Vector
|
Construct size (kb): 10.10000038146973
|
|
Insert
|
DNA: genomic Insert lengths(kb): 1.799999952316284 Gene product: heat-labile enterotoxin [lt] Target Gene: LT toxin, heat-labile enterotoxin
|
|
Shipping Information
|
Shipped: Freeze dried phagemid in E. coli
|
|
Comments
|
The insert contains internal HincII, EcoRI and HindIII sites. The plasmid exists in DH1 as a multimer. Digestion of the plasmid with HindIII yields a 0.85 kb fragment containing nucleotide sequence for the entire B subunit and about one-third of the A subunit. This is a deletion mutant of EWD022 and seems to contain the minimum coding sequence to obtain functional proteins. This expresses proteins of 22.5 kDa and 11.5 kDa, having both LT and adenylate cyclase-activating activity.
|
|
Classification
|
Enterobacteriaceae, Escherichia
|
|
References
|
Dallas WS, et al. Cistrons encoding Escherichia coli heat-labile toxin. J. Bacteriol. 139: 850-858, 1979. PubMed: 383697
U.S. Food & Drug AdministrationEnterotoxigenic Escherichia coli: Heat-stabile enterotoxin (human), heat-stabile enterotoxin (porcine), and heat-labile enterotoxinIn: U.S. Food & Drug AdministrationBacteriological analytical manual8th ed.Gaithersburg, MDAOAC Internationalpp. 24.12-24.14, 1995
AOAC International Detection of Escherichia coli producing heat-labile enterotoxin. DNA colony hybridization method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 984.34.
Guzman-Verduzco LM, Kupersztoch YM. Fusion of Escherichia coli heat-stable enterotoxin and heat-labile enterotoxin B subunit. J. Bacteriol. 169: 5201-5208, 1987. PubMed: 3312169
|