Human respiratory syncytial virus (ATCC® VR-26)

Classification: Paramyxoviridae, Pneumovirus  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

View Permits

Classification Paramyxoviridae, Pneumovirus
Deposited As Respiratory syncytial virus
Agent Human respiratory syncytial virus
Strain Long
Applications
Testing virucides
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-26 Micrograph
Comments Non-pathogenic for mouse or guinea pig. Essentially identical to Chimpanzee coryza agent. Prototype strain for subgroup A.
Effect on Host
CPE, syncytia formation, rounding, sloughing
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-12 days
Effect on Host
CPE, syncytia formation, rounding, sloughing
Name of Depositor RM Chanock, National Institute of Allergy and Infectious Diseases
Special Collection NCRR Contract
Source 17-month-old male with pneumonia, Maryland, 1956
References

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).

Blount RE Jr, et al. Recovery of cytopathogenic agent from chimpanzees with coryza. Proc. Soc. Exp. Biol. Med. 92: 544-549, 1956.

Chanock R, et al. Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). I. Isolation, properties and characterization. Am. J. Hyg. 66: 281-290, 1957. PubMed: 13478578

Walpita P, et al. Distinguishing between respiratory syncytial virus subgroups by protein profile analysis. J. Clin. Microbiol. 30: 1030-1032, 1992. PubMed: 1572961

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation