Hepatitis A virus (ATCC® VR-2093)

Classification: Picornaviridae, Hepatovirus  /  Product Format: frozen 1.0 mL

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Classification Picornaviridae, Hepatovirus
Deposited As Hepatitis A
Agent Hepatitis A virus
Strain HM 175 (uncloned)
Applications
testing virucides
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images ATCC VR-2093 images
Comments This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims.
Effect on Host
CPE, cell rounding and deterioration. The CPE may not appear until near the end of the incubation period.
Recommended Host
FRhK-4 cells (ATCC® CRL-1688™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 21-28 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, cell rounding and deterioration. The CPE may not appear until near the end of the incubation period.
Name of Depositor National Institutes of Health (NIH)
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Source Human stool specimens from outbreak of virus, Australia
References

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).

Daemer RJ, et al. Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect. Immun. 32: 383-393, 1981. PubMed: 6260685

Feinstone SM, et al. Isolation of hepatitis A virus strain HM-175. US Patent 4,532,215 dated Jul 30 1985

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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