Trichophyton mentagrophytes (Robin) Blanchard, anamorph (ATCC® MYA-4439)

Alternate State: Arthroderma benhamiae Ajello et Cheng, teleomorph, Arthroderma vanbreuseghemii Takashio, teleomorph  /  Strain Designations: MRL 1957  /  Product Format: frozen

Permits and Restrictions

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Strain Designations MRL 1957
Synonym Trichophyton interdigitale
Alternate State Arthroderma benhamiae Ajello et Cheng, teleomorph, Arthroderma vanbreuseghemii Takashio, teleomorph
Application
Susceptibility testing
Antifungal susceptibility testing RefGhannoum MA, et al. Interlaboratory study of quality control isolates for a broth microdilution method (modified CLSI M38-A) for testing susceptibilities of dermatophytes to antifungals. J. Clin. Microbiol. 44: 4353-4356, 2006. RefEspinel-Ingroff A, et al. Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J. Clin. Microbiol. 43: 5243-5246, 2005.
Biomedical Research and Development Material
Biosafety Level 2
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Comments
the most common cause of athlete's foot
keratinophilic dermatophyte
Morphology After 10 days on Emmons' medium colonies powdery to floccose, cream-coloured to yellowish-buff; reverse ochre to red-brown. Macroconidia sparse, 3-8-celled, clavate to cigar-shaped. Microconidia spherical, sessile, arranged in dense, grape-like clusters or alongside the hyphae.
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

AGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAGCGCGCAGGCCGGAGGCTGGCCCCCCACGATAGGGCCAAACGTCCGTCAGGGGTGAGCAGATGTGCGCCGGCCGTACCGCCCCATTCTTGTCTACATTACTCGGTTGCCTCGGCGGGCCGCGCTCTCCCAGGAGAGCCGTTCGGCGAGCCTCTCTTTAGTGGCTAAACGCTGGACCGCGCCCGCCGGAGGACAGACGCAAAAAAATTCTTTCAGAAGAGCTGTCAGTCTGAGCGTTAGCAAGCAAAAATCAGTTAAAACTTTCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGCATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAGCCCCTCAAGCCCGGCTTGTGTGATGGACGACCGTCCGGCGCCCCCGTCTTTGGGGGTGCGGGACGCGCCCGAAAAGCAGTGGCCAGGCCGCGATTCCGGCTTCCTAGGCGAATGGGCAACAAACCAGCGCCTCCAGGACCGGCCGCCCTGGCCTCAAAATCTGTTTTATACTTATCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCG

Morphology After 10 days on Emmons' medium colonies powdery to floccose, cream-coloured to yellowish-buff; reverse ochre to red-brown. Macroconidia sparse, 3-8-celled, clavate to cigar-shaped. Microconidia spherical, sessile, arranged in dense, grape-like clusters or alongside the hyphae.
Name of Depositor MA Ghannoum
Special Collection ATCC
Chain of Custody
ATCC <-- Ghannoum
Isolation
human toenail, USA
Cross References

Nucleotide (GenBank) : FJ746658 ITS including 5.8S rRNA gene

References

Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M38-A.

Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi: Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M38-A2.

Ghannoum MA, et al. Interlaboratory study of quality control isolates for a broth microdilution method (modified CLSI M38-A) for testing susceptibilities of dermatophytes to antifungals. J. Clin. Microbiol. 44: 4353-4356, 2006.

Shehata AS, et al. Comparison between the standardized Clinical and Laboratory Standards Institute M38-A2 method and a 2,3-bis(2-methoxy-4-nitro-5-[(sulphenylamino)carbonyl]-2H-tetrazolium hydroxide- based method for testing antifungal susceptibility of dermatophytes. J. Clin. Microbiol. 46: 3668-3671, 2008.

Espinel-Ingroff A, et al. Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J. Clin. Microbiol. 43: 5243-5246, 2005.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation