Geobacillus stearothermophilus (Donk) Nazina et al. (ATCC® 7953)

Type Strain: no  /  Biosafety Level: 1

Deposited As Bacillus stearothermophilus Donk
Gas sterilization control
Steam sterilization control
Sterility assurance
Sterilization control
Pharmaceutical and Personal Care
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain no
This strain is recommended by ATCC for use in the tests described in military specification MIL S-36586A and U.S. Pharmacopeia, 23rd rev., <1211>, pp. 1976-1981, 1995 where only the taxon is specified.
Stated to grow at 35°C
The ATCC lab was unable to verify growth at 35°C.
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 55°C
Atmosphere: Aerobic
Name of Depositor CP Hegarty

Smith NR, et al. Aerobic spore forming bacteria. U.S. Dep. Agric. Monogr. 16: 1-148, 1952.

Arch. Biochem. Biophys. 54: 154-161, 1955.

British Pharmacopoeia Commission Methods of sterilization. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVIII, 2003

European Pharmacopoeia Commission Biological indicators of sterilization. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.2, 1997

U.S. Pharmacopeia USP Monographs: Biological Indicator for Steam Sterilization, Paper Carrier. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

Terminal Sterilization and Sterilization Indicators. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.15.

Sterilization of health care products--Biological indicators--Part 3: Biological indicators for moist heat sterilization.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11138-3:1995.

Sterilization of health care products --- Biological indicators, Part 3: Biological indicators for moist heat sterilization processes. London, UK:British Standards Institution;British Standard BS EN ISO 11138-3:2006.

Sterilization of health care products --- Biological indicators, Part 5: Biological indicators for low-temperature steam and formaldehyde sterilization processes. London, UK:British Standards Institution;British Standard BS EN ISO 11138-5:2006.

Cross References

Nucleotide (GenBank) : L13418 Bacillus stearothermophilus maltose permease (malA) gene, complete cds.

Nucleotide (GenBank) : M16814 B.stearothermophilus small acid-soluble spore protein (B-type SASP) gene, complete cds.

Nucleotide (GenBank) : M86508 Bacillus stearothermophilus proton glutamate symport protein (gltP) gene, complete cds.

Nucleotide (GenBank) : M13061 B.stearothermophilus SASP-1 gene encoding a small acid-soluble spore protein, complete cds.

Nucleotide (GenBank) : AF184067 Bacillus stearothermophilus MetS (metS) gene, partial cds; transition state regulator (abrB) gene, complete cds; and yabC pseudogene, partial sequence.

Nucleotide (GenBank) : U18943 Bacillus stearothermophilus mannitol transport protein (MtlA), putative transcriptional regulator (MtlR), mannitol enzyme IIA (MtlF) and mannitol-1-phosphate dehydrogenase (MtlD) genes, complete cds.

Basic Documentation
  1. ATCC item no. 7953 growth on blood agar
    Yes, we have been able to grow this one on blood agar plates
    Date Updated: 12/5/2013