Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers (ATCC® 25285)

Strain Designations: VPI 2553 [EN-2; NCTC 9343]  /  Type Strain: yes  /  Biosafety Level: 2

Permits And Restrictions

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Strain Designations VPI 2553 [EN-2; NCTC 9343]
Media testing
Quality control strain
Susceptibility testing
Quality control strain for BBL and MicroScan® products
Pharmaceutical and Personal Care
Appendix abscess
Type Strain yes
Biosafety Level 2
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Genome Sequenced Strain

Bacteroides fragilis NCTC 9343, complete genome

Type Strain yes
Genome sequenced strain
Medium ATCC® Medium 1490: Modified chopped meat medium
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Name of Depositor WE Moore
Chain of Custody
ATCC <-- WE Moore <-- A Sonnenwirth EN-2 <-- NCTC 9343 <-- L Garrod
Appendix abscess

Cato EP, Johnson JL. Reinstatement of species rank for Bacteroides fragilis, B. ovatus, B. distasonis, B. thetaiotaomicron, and B. vulgatus: Designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides thetaiotaomicron (Distaso) Castellani and Chalmers. Int. J. Syst. Bacteriol. 26: 230-237, 1976.

Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M11-A7.

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980.

Takahata M, Nishino T. Antibacterial activities of tosufloxacin against anaerobic bacteria and the electron micrograph of its bactericidal effects. Chemotherapy 43: 153-158, 1997. PubMed: 9142454

BD Crystal ANR QC Set. BD Becton Dickinson; Crystal

MicroScan Rapid Anaerobe ID QC Set. Dade/Siemens.

Sensititre™ Anaerobe MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.

Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A.

Medical microbiology--Culture media -- Part 4: Transport systems for specimens containing bacteria. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-4: 2003, 2003

Cross References

Nucleotide (GenBank) : X83935 B.fragilis 16S rRNA (ATCC 25285T).

Nucleotide (GenBank) : AX110196 Sequence 929 from Patent WO0123604.

Nucleotide (GenBank) : AF280705 Bacteroides fragilis isolate ATCC 25285 RecA (recA) gene, partial cds.

Nucleotide (GenBank) : U05887 Bacteroides fragilis ATCC 25285 beta-lactamase (cepA-85) gene, complete cds.

Nucleotide (GenBank) : AF280642 Bacteroides fragilis isolate ATCC 25285 glutamine synthetase (glnA) gene, partial cds.

Nucleotide (GenBank) : CR626927 Bacteroides fragilis NCTC 9343, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Commercially prepared medium for Bacteroides fragilis (ATCC® 25285)

    Date Updated: 7/25/2012

  2. ATCC item no. 25285 growth characteristics

    Date Updated: 11/5/2013