RNA from Human respiratory syncytial virus strain 18537 (ATCC® VR-1580D)

Organism: Human respiratory syncytial virus  / 

Permits and Restrictions

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Product Format frozen
Total genomic RNA from infected cells, 100 µL per vial, shipped frozen
Storage Conditions -70°C or colder
The vapor-phase of liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).
Applications
It is suitable for use in reverse transcriptase-polymerase chain reaction (RT-PCR) for viral gene products and other molecular virology applications.
Comments
This product is a preparation of viral nucleic acids, including virus genomic RNA, in total RNA isolated from HEp-2 cells (ATCC® CCL-23™) infected with Human respiratory syncytial virus (HRSV) strain 18537 (ATCC® VR-1580™). It is supplied at 100 ul per vial, shipped frozen. It is suitable for use in reverse transcriptase-polymerase chain reaction (RT-PCR) for viral gene products and other molecular virology applications.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications
  1. Concentration is verified as ≥ 1µg/mL by Ribogreen® assay.
  2. Integrity is inferred from observation of intact RT-PCR product on a 1.2% agarose gel, visualized by ethidium bromide staining.
  3. Functional activity is demonstrated by RT-PCR amplification of ≥ 500 bp amplicon dilutable ≥ 10 fold.
  4. Identity is confirmed by sequence analysis of the RT-PCR amplicon.
  5. Virus inactivation is verified by the incubation of 10% of total yield on susceptible host cells followed by evaluation for cytopathic effect.
  6. Purity is confirmed by OD260/OD280 ratio (≥ 1.8) of bulk RNA prior to dilution for product filling.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation