DNA from Vaccinia virus strain Modified Vaccinia Virus Ankara (MVA) (ATCC® VR-1508D)

Organism: Vaccinia virus  / 

Permits and Restrictions

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Product Format frozen

PRODUCT INFORMATION:

Total DNA from infected cells, >1 µg/mL in 1X TE buffer

100 µL per vial, shipped frozen

Storage Conditions -70°C or colder
Applications
It is suitable for use in PCR and other molecular virology applications.
Comments
Vaccinia virus DNA, in total DNA isolated from BHK-21 cells infected with Vaccinia Virus, strain Modified Vaccinia Virus Ankara (MVA). This product was prepared using methods known to inactivate viruses. It is suitable for use in PCR and other molecular virology applications.
Biosafety Level 1
Quality Control Specifications
1. Store at -20°C or colder. -70°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).

2. Concentration is verified as >1 µg/mL DNA by Picogreen® assay.

3. Purity is confirmed by OD260/OD280 ratio (>1.7) of bulk DNA before dilution for product filling.

4. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis of 20 µl of product on a 0.8% agarose gel, visualized by Ethidium bromide staining.

5. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.

6. Identity is confirmed by sequencing of approximately 300 bp to 1Kb of the PCR amplicon.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation