Human genomic DNA (HCC2218) (ATCC® CRL-2343D)

Organism: Homo sapiens, human  / 

Permits and Restrictions

View Permits

Product Format frozen Approx.10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level 1
Introduction

Cell Line Description:

The HCC2218 cell line was initiated on April 10, 1996, and took 6 months to establish. HCC2218 was derived from a female whose family members included several members with cancer, four lung cancer patients (two of them, non-smokers), two breast cancer patients (one of them also had lung cancer) and one colon cancer patient. HCC2218 is a poorly differentiated cell line derived from the primary tumor of an invasive ductal carcinoma with metastases in 42/43 lymph nodes. The cells are highly positive for expression of Her2/neu, and are also positive for expression of p53. HCC2218 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +). DNA was isolated from an EBV-transformed lymphoblastoid cell line, HCC2218 BL, from the same patient is available as ATCC CRL-2363D.

Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation